Abstract
3,4-Dihydro-6,7-dimethoxy-4-methyl-3-oxo-qunoxaline-2-carbonyl chloride was found to be a highly sensitive fluorescence derivatization reagent for primary and secondary alcohols in high-performance liquid chromatography. Its reactivity was investigated for benzyl alcohol, n-hexanol and cyclohexanol. The reagent reacts with the alcohols in benzene to produce the corresponding fluorescent esters, which can be separated on a reversed-phase column, YMC Pack C 8, with aqueous 70% (v/v) methanol; the detection, limits for the alcohols were 2–3 fmol for an injection volume of 10 μ1. The reagent also reacts with hydroxysteroids with primary and/or secondary alcoholic group(s) to form fluorescent derivatives. Tertiary alcohols, hydroxycarboxylic acids and phenols do not give fluorescent derivatives under these conditions.
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