Abstract
Publisher Summary The resulting hybrid proteins (conjugates) can be used as an agent to select resistant cell variants that are unable to bind, take up, or process the growth factor. The general strategy of this conjugate technique has been described. Two different cross-linking techniques, both through disulfide linkages, are described: one that modifies carboxyl groups and one that modifies amino groups of growth factors such as epidermal growth factor (EGF) and insulin. Procedures for isolating conjugate-resistant cell variants are also described. The conjugates of peptide growth factors and toxins not only allow selection of cell variants specifically related to the biological activity of each growth factor but also provide variants with a wide array of properties. The reasons for this variety may be 2-fold: the properties of the conjugates and the nature of the target cell's endocytotic mechanisms. Possible modification of the method and various applications has been discussed in detail.
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