Abstract
Background and Aims: The dysmorphism of oocytes either extra or intracytoplasmic could decrease viability and developmental competence of oocytes. There are several reports describing that specific dysmorphisms are related with decrease in fertilization and embryo competence. The aim of this research is to investigate fertilization and embryo quality of specific dysmorphic mature oocytes obtained from ICSI. Method: A retrospective analysis was performed in 183 patients with 1700 mature oocytes. Mature oocytes were classified as normal morphology (control), oocytes with intra-cytoplasmic dysmorphism (fragile oocytes, inclusion bodies, vacuoles, mild granularity of cytoplasm, severe granularity of cytoplasm, and central granulation), and oocytes with extra-cytoplasmic dysmorphism (granulation in perivitelline space, polar body fragmentation, large perivitelline space, and abnormal zona pellucida). All oocytes were inseminated with ICSI. Oocytes were cultured and scored on day 3 then some of them were transferred. The rest of oocytes were extended culture until day 5. Day 3 embryos were classified based on grading by Kamardi et al., 2021. Grade 1 and 2 embryos are considered good quality embryos. Day 5 embryos were classified based on modified grading by Gardner and Schoolcraft., 1999. Results: Overall, fertilization rate was significantly lower in the dysmorphic oocytes compared to control (P<0.01). Good quality embryo development on day 3 was significantly lower in oocytes with severe granularity of cytoplasm compared to control (P<0.05). Looking at embryo development on day 5, oocytes with inclusion bodies and central granulation had significantly lower on good quality blastocyst (P<0.05). Conclusion: The fertilization potential of dysmorphic oocytes in our study was lower compared to normal morphology oocytes, however, only severe granularity of cytoplasm, inclusion bodies and central granulation related with poor embryo quality on day 3 and day 5. In this study, we were able to define several specific of oocyte dimorphism related with lower fertilization and embryo quality.
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