Abstract
Abstract The ability of alkalotolerant bacterium Zacillus FTU to maintain intracellular pH was studied in the wide range of extracellular pH: 6.5–11, by means of 31P NMR. Bacillus FTU is an obligatory aerobic microorganism, possessing a high speed of oxygen consumption, that is why it was especially important to provide sufficient oxygen supply during the entire period of measurement. We have worked out a special glass filter insert for the NMR sample tube which allowed for uniform air supply in the cell suspension. The viability of the cells was checked by ATP level analysis Since in the case of Bacillus FTU ATP content if highly sensitive to the quantity of oxygen. The endogeneous P1 was used as a pH indicator. The level of Pi is extremely high in Bacillus FTU-around 70–30 mM, that's why it was possible to use relatively low concentration of cells in the suspension to obtain sufficient NMR sensitivity (approximately 5–7; of intracellular volume). Special procedure was employed to obtain a calibration cu...
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