311 SECRETED FACTORS FROM PERITUMOR ADIPOSE TISSUES OF CLEAR CELL RENAL CELL CARCINOMA INCREASED THE MOTILITY OF HUMAN CCRCC CELL LINE CAKI-2 VIA ENHANCEMENT OF WNT SIGNALING

Abstract

You have accessJournal of UrologyKidney Cancer: Basic Research (II)1 Apr 2013311 SECRETED FACTORS FROM PERITUMOR ADIPOSE TISSUES OF CLEAR CELL RENAL CELL CARCINOMA INCREASED THE MOTILITY OF HUMAN CCRCC CELL LINE CAKI-2 VIA ENHANCEMENT OF WNT SIGNALING Achim Lusch, Vien Nguyen, Christopher Blair, Molly Baker, Victor Huynh, Xiaolin Zi, and Jaime Landman Achim LuschAchim Lusch Orange, CA More articles by this author , Vien NguyenVien Nguyen Orange, CA More articles by this author , Christopher BlairChristopher Blair Orange, CA More articles by this author , Molly BakerMolly Baker Orange, CA More articles by this author , Victor HuynhVictor Huynh Orange, CA More articles by this author , Xiaolin ZiXiaolin Zi Orange, CA More articles by this author , and Jaime LandmanJaime Landman Orange, CA More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1695AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Perinephric adipose tissue (PAT) is metabolically active and manifests endocrine function. We evaluated the interaction between PAT and clear cell renal cell cancer (ccRRC) cells. METHODS PAT was collected from 49 patients (27 clear cell, 7 chromphobe, 1 papillary, 3 uncharacterized RCCs, and 11 benign pathology). PAT was harvested from patients undergoing renal surgery and cultured for 24 hours to generate conditioned media (CM). The effect of PAT CM on the proliferation and migration of ccRCC cell line Caki-2 was measured by MTT assay and Boyden chamber cell migration assay, respectively. In addition, Wnt/β-Catenin activity in PAT CM was examined as induced levels of β-Catenin. The proliferative and migratory responses are expressed as a stimulation index (SI) calculated by dividing the mean number of proliferative or migratory cells of PAT CM-stimulated wells by those of non-stimulated wells. Wnt response is similarly expressed as a ratio of mean Wnt activity of PAT CM-stimulated cells divided by that of non-stimulated cells. RESULTS PAT CMs from pT3 ccRCC patients resulted in a significant increase of CaKi-2 cell migration (mean SI and standard deviation, 1.80±0.52, P<0.05) compared to those at pT1 ccRCC (1.36±0.71, P>0.05) and pT2 (1.60±1.22, P>0.05), respectively. Conversely, the mean proliferative SIs of PAT CMs from ccRCC patients decreased as clinical stage advanced (pT1 versus pT3, 1.28± 0.37 vs. 0.83± 0.08). PAT CMs from patients with benign pathology has no significant effect on proliferation and migration. PAT CMs from chromophobe RCC also significantly increased the migration of Caki-2 (migratory SI, 1.96 ± 1.01) with no effect on proliferation (SI, 1.02 ± 0.50). High Fuhrman Grade is associated with increased migration by PAT CMs. Increased tumor sizes were inversely associated with proliferative SIs (correlation coefficient, -0.45). The increased migration of Caki-2 cells is associated with enhanced Wnt activity in PAT CMs. CONCLUSIONS We demonstrate a novel interaction between PAT and RCC which may facilitate understanding of cancer cell migration and metastasis in RCC patients. Further analysis is in progress. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e126 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Achim Lusch Orange, CA More articles by this author Vien Nguyen Orange, CA More articles by this author Christopher Blair Orange, CA More articles by this author Molly Baker Orange, CA More articles by this author Victor Huynh Orange, CA More articles by this author Xiaolin Zi Orange, CA More articles by this author Jaime Landman Orange, CA More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...