3′:5′-Cyclic GMP in the yeast Saccharomyces cerevisiae at different metabolic conditions

Abstract

cGMP is characterized as undetectable in yeast [(1986) Yeast Cell Biology, UCLA Symp. Mol. Cell Biol. (Hicks, J. ed.) p.495], though in many organisms it contributes specifically to the regulation of metabolism. Here, we detected cGMP, using radioactive labeling and RIA techniques, after extraction of the cells with mol/1 HClO 4 at 37°C. The cGMP 0.015-fold cAMP, about 3 times higher with exponentially growing cells than with pressed baker's yeast, and depends on glucose and O 2 supply. The PDE inhibitors DMX and IBMX induce in growing cells an additional increase of the cGMP level, without similar effects on cAMP.