Abstract
Objective: Present study aims to investigate 28-homocastasterone (28-HC) influences on testicular tissue in the normal and diabetic rat.Methods: Induction of diabetes was achieved by single peritoneal injection of streptozotocin (60 mg/kg b. wt) followed by 28-HC (100 µg/150 gm body weight) administration by oral gavage for 15 consecutive days to experimental rats. 3β-hydroxysteroid dehydrogenase, 17β-hydroxysteroid dehydrogenase activities, testosterone level, Liver X Receptor (LxR) mRNA expression, malondialdehyde (MDA), reduced glutathione (GSH) and testis histology was analysed.Results: Increased cholesterol level, 3β-hydroxysteroid dehydrogenase (3βHSD), 17β-hydroxysteroid dehydrogenase (17βHSD) activities, testosterone level with significant elevation of LxR-α and β mRNA expression in treated rat testis. A significant reduction found inMDA and increased reducedGSH along with improved testicular architecture was observed.Conclusion: In the present study demonstrated that 28-HC induced 3βHSD, 17βHSD enzyme activity and testosterone level, thus indicative of steroidogenic potential and capable of transactivating LxR-α and β molecular operative in rat testicular tissue.
Highlights
Mammalian testicular biology is complex next to the nerves system
Studies with phytosteroid showed testicular Liver X Receptor (LxR) transactivations resulted to modulation of several testicular functions, such as steroidogenesis, cholesterol homeostasis and proliferative apoptosis balance affected through regulation of gene expression [2]
Fatim-Zorah et al cell culture studies reported that LxR-α isoform regulates sertoli cell cholesterol metabolism, whereas LxR-β regulates leyding cell cholesterol metabolism and testosterone biosynthesis, in contrast, LxRs knockout mice based study the independent role of LxRs in testicular tissue was reported that the LXR-α is highly expressed in the leydig cells and LXR-β in sertoli cells and both isoforms are found to be expressed in the germ cells [2]
Summary
Mammalian testicular biology is complex next to the nerves system. There are two biosynthetic events significantly contribute to male reproductive processes are steroidogenesis and spermatogenesis, under control of endocrine and exocrine factors peptides or steroids. Testicular steroid metabolism are complex biochemical pathway can be influenced by biologically active dietary signalling factors present at low abundance such as phytosteroids, phytohormones, polyphenols and terpenoids are being considered for their role in human health and disease recently [1]. Studies with phytosteroid showed testicular Liver X Receptor (LxR) transactivations resulted to modulation of several testicular functions, such as steroidogenesis, cholesterol homeostasis and proliferative apoptosis balance affected through regulation of gene expression [2]. Which isoform of LxRs and its specific types of ligands (oxysterol) responses for testicular testosterone production was unclear
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