Abstract
Publisher Summary This chapter describes the quantitation of anethole dithiolethione using high performance liquid chromatography (HPLC) with electrochemical detection. The chapter discusses a highly reproducible and effective method to measure anethole dithiolethione (ADT) in cells as well as in aqueous solutions. The present method is 50-fold more sensitive than previously reported for fluorescence detection of ADT following HPLC separation. In addition, the extraction method described in the chapter removes nearly 100% of the ADT from cellular or aqueous samples. In addition to the increased sensitivity of the EC method, this new method also allows measurement of any redox products of ADT. Because ADT has a similar structure to lipoic acid, ADT may interact with other cellular thiols in a manner similar to lipoic acid. When lipoic acid enters the cell, the disulfide bond is reduced to form dihydrolipoic acid. It is not yet known whether ADT is metabolized in a similar manner when it is incubated with Wurzburg cells.
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