Abstract
This chapter discusses the molecular analysis of plant transpiration efficiency (W). The chapter discusses two routes to conduct the molecular analysis of genetic variation in transpiration efficiency. One route is to search for DNA polymorphisms associated with variation of transpiration efficiency among lines or ecotypes of the species of interest. An alternative is to screen a population of artificially mutagenized seedlings for mutations affecting transpiration efficiency and proceed to gene mapping and complementation. The chapter illustrates the analysis of DNA polymorphisms associated with variation of carbon isotope discrimination in Arabidopsis thaliana. The classical technique for detecting DNA polymorphisms is the analysis of Restriction Fragment Length Polymorphisms (RFLPs). RFLPs detect differences in the length of specific DNA fragments after digestion of genomic DNA with sequence-specific restriction endonucleases. The chapter describes the genetic analysis of the link between carbon isotope discrimination and associated traits. RFLP analysis of carbon isotopic discrimination can be extended to other associated traits with only the cost of more extensive phenotypic screening, with the same molecular data being used for all traits.
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