Abstract

Among various genomic features in the blood, methylation pattern could be useful not only in determining whether cancer is present, but in revealing its origin. The purposes of this prospective study were to evaluate the diagnostic performance of methylation sensitive restriction enzyme digestion followed by sequencing method (MRE-seq) using cell-free DNA (cfDNA) in the diagnosis of cancer and to investigate tissue-of-origin (TOO) using deep neural network (DNN).

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