Abstract
Many different methods have been described for the extraction of tocopherols from animal and plant tissues. Most of these procedures fall into one of two groups—the Soxhlet extraction of freeze-dried tissue and direct extraction of the fresh tissue by homogenization in the extracting solvent. Soxhlet extraction of freeze-dried samples with acetone has been shown to be superior to other available methods for the extraction of tocopherols from animal tissues, and the extraction of freeze-dried plant tissues with chloroform has been shown to be very efficient. However, the difficulties often associated with the freeze-drying of plant tissues casts doubt on the general application of the procedure to these tissues. Instead, direct extraction of the fresh tissue is preferred. The choice of solvents for the extraction of plant tissues has presented some problems. Although ethanol has been used, further use of this solvent must be excluded by the fact that even boiling ethanol does not immediately destroy α-tocopherol oxidase activity in plant homogenates.
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