23 ANGIOTENSIN II RECEPTOR BLOCKER, OLMESARTAN PRESERVES BLADDER BLOOD FLOW AND OXIDATIVE STRESS, AND IMPROVES BLADDER DYSFUNCTION IN THE SPONTANEOUSLY HYPERTENSIVE RAT

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You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research (1)1 Apr 201323 ANGIOTENSIN II RECEPTOR BLOCKER, OLMESARTAN PRESERVES BLADDER BLOOD FLOW AND OXIDATIVE STRESS, AND IMPROVES BLADDER DYSFUNCTION IN THE SPONTANEOUSLY HYPERTENSIVE RAT Shogo Shimizu, Motoaki Motoaki, Harunori Oiwa, Fumiya Ohmasa, Panagiota Tsounapi, Ryo Oikawa, Yukako Kinoshita, and Shuhei Tomita Shogo ShimizuShogo Shimizu Yonago, Japan More articles by this author , Motoaki MotoakiMotoaki Motoaki Yonago, Japan More articles by this author , Harunori OiwaHarunori Oiwa Yonago, Japan More articles by this author , Fumiya OhmasaFumiya Ohmasa Yonago, Japan More articles by this author , Panagiota TsounapiPanagiota Tsounapi Yonago, Japan More articles by this author , Ryo OikawaRyo Oikawa Yonago, Japan More articles by this author , Yukako KinoshitaYukako Kinoshita Yonago, Japan More articles by this author , and Shuhei TomitaShuhei Tomita Yonago, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1398AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Hypertension (HT) is one of the risk factors for lower urinary tract symptoms (LUTS). Anti-hypertensive drugs, angiotensin II type I (AT-1) receptor blockers (ARBs) is reported to ameliorate urodynamic parameters in human clinical or animal studies. In the present study, we investigated the effect of an ARB, olmesartan on bladder blood flow (BBF), oxidative stress and urodynamic parameters in the spontaneously hypertensive rat (SHR). METHODS Twelve-week-old male SHRs were treated with olmesartan (0, 1 or 3 mg/kg/day per orally (p.o.)) (SHR, Olm1, Olm3) or nifedipine (30 mg/kg/day p.o.) (Nif) for 6 weeks. Wistar rats (Cont) were used as normotensive age-matched controls. The effects of olmesartan or nifedipine on blood pressure (BP), BBF and urodynamic parameters were measured using tail cuff method, hydrogen clearance method, metabolic cage and cystometry, respectively. The tissue bladder level of malondialdehyde (MDA) was measured by colorimetric assay. The tissue levels of the nuclear factor erythroid 2-related factor 2 (Nrf2) and nerve growth factor (NGF) in the bladder were measured by ELISA. Immunoreactivity of 4-hydroxy-2-nonenal (4-HNE), Nrf2 and NGF was examined by immunohistochemistry in the bladder. RESULTS The SHR showed significant increase in BP, micturition frequency, and MDA, Nrf2 and NGF expressions in the bladder, and decrease in BBF and single voided volume compared to the control group. Although both treatment with olmesartan and nifedipine significantly improved the BP in the SHR, only olmesartan significantly ameliorated these parameters compared to the SHR in a dose dependent manner. The immunoreactivities of 4-HNE, Nrf2 and NGF in the SHR urothelium and vascular vessel were increased compared to the control group. Treatment with olmesartan decreased the expressions of 4-HNE, Nrf2 and NGF in the bladder. CONCLUSIONS Our data suggest that BP, BBF and oxidative stress affect bladder function, and that olmesartan significantly ameliorated bladder dysfunction through the recovery of the BP, BBF and oxidative stress. In conclusion, ARB could be first line treatment of HT patients with LUTS. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e9 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Shogo Shimizu Yonago, Japan More articles by this author Motoaki Motoaki Yonago, Japan More articles by this author Harunori Oiwa Yonago, Japan More articles by this author Fumiya Ohmasa Yonago, Japan More articles by this author Panagiota Tsounapi Yonago, Japan More articles by this author Ryo Oikawa Yonago, Japan More articles by this author Yukako Kinoshita Yonago, Japan More articles by this author Shuhei Tomita Yonago, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...