2278 RE-ENDOTHELIALIZATION OF ACELLULAR KIDNEY SCAFFOLDS FOR WHOLE ORGAN ENGINEERING

Abstract

You have accessJournal of UrologyTransplantation & Vascular Surgery: Renal Transplantation, Vascular Surgery II1 Apr 20122278 RE-ENDOTHELIALIZATION OF ACELLULAR KIDNEY SCAFFOLDS FOR WHOLE ORGAN ENGINEERING In Kap Ko, Sayed Hadi Mirmalek Sani, Shay Soker, Tamer Aboushwareb, Anthony Atala, and James Yoo In Kap KoIn Kap Ko Winston Salem, NC More articles by this author , Sayed Hadi Mirmalek SaniSayed Hadi Mirmalek Sani Winston Salem, NC More articles by this author , Shay SokerShay Soker Winston Salem, NC More articles by this author , Tamer AboushwarebTamer Aboushwareb Winston Salem, NC More articles by this author , Anthony AtalaAnthony Atala Winston Salem, NC More articles by this author , and James YooJames Yoo Winston Salem, NC More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.2456AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The lack of transplantable allografts for renal failure has prompted the development of scaffold design and cell isolation techniques in order to re-create an engineered whole kidney. Decellularized solid organ xenografts such as the kidney hold great promise due to the maintenance of structural integrity while removing all cellular components. However, acelluar kidney vasculature needs an endothelial cell lining to avoid blood clotting and thrombus formation when transplanted into a living recipient. In order to maintain blood circulation and induce renal functions, re-endothelialization of acellular scaffold is critical. METHODS In this study, we describe an endothelial cell seeding method that permits effective endothelial cell coating on the vascular walls of the decellularized porcine kidney scaffolds. We conducted MS1 endothelial cell seeding of acellular porcine renal scaffold using a combination method (static and ramping perfusion method). RESULTS We showed that combined MS1 seeding facilitated well-organized endothelium on the decellularized blood vessels, confirmed by H&E staining and SEM analysis. Organized endothelial cells were shown on the artery, vein, and smaller blood vessels without apparent clogging of cells at the capillary structures in the cortex. Vascular tree imaging demonstrated that the endothelium on the arterial system of the kidney is well preserved and covered with viable endothelial cells, confirmed by GFP expression on continuous arterial branches of the entire kidney. Furthermore, MS1 seeded scaffold displayed significant inhibition of platelet adhesion on cell-attached surfaces as compared to the strong platelets deposition found on the unseeded scaffolds. CONCLUSIONS Using a combined static and ramping perfusion seeding method proved to be a viable method for endothelial cells seeding. Future work will be aimed at improving cell adhesion as well as increasing cell numbers. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e919 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information In Kap Ko Winston Salem, NC More articles by this author Sayed Hadi Mirmalek Sani Winston Salem, NC More articles by this author Shay Soker Winston Salem, NC More articles by this author Tamer Aboushwareb Winston Salem, NC More articles by this author Anthony Atala Winston Salem, NC More articles by this author James Yoo Winston Salem, NC More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...