Abstract
Vascular endothelial cell is exposed to shear stress by vascular flow, extracellular temperature and other physical stimulus in vitro. Nitric oxide produced by endothelial nitric oxide synthase(NOS3) in vascular endothelial cell vasodilate blood vessel and relates to vascular flow regulation. It has been reported that shear stress induced to cultured vascular endothelial cell phosphorylates and activates intracellular NOS3 and leads to an increase in nitric oxide production. It had been observed that blood vessel and skin temperature decrease and then change irregularly when local is exposed in cold water, but the mechanism is not clear. In the present study, we stimulated HMVECs by low temperature on static or shear stress condition and investigates NOS3 activity with SDS-PAGE, Western blotting and immunostaining.
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