Abstract
Novel point-of-care compatible methods such as colorimetric assays have become increasingly important in the field of early pathogen detection. A simple and hand-held prototype device for carrying out DNA-amplification assay based on plasmonic nanoparticles in the colorimetric detection is presented. The low-cost device with two channels (sample and reference) consists of two spectrally different light emitting diodes (LEDs) for detection of the plasmon shift. The color change of the gold-nanoparticle-DNA conjugates caused by a salt-induced aggregation test is examined in particular. A specific and sensitive detection of the waterborne human pathogen Legionella pneumophila is demonstrated. This colorimetric assay, with a simple assay design and simple readout device requirements, can be monitored in real-time on-site.
Highlights
In the field of pathogen diagnostics, innovative point-of-care compatible methods such as colorimetry have become increasingly important in recent years
The development of a simple, hand-held, and low-cost device for carrying out colorimetric detection based on plasmonic nanoparticles and a DNA-amplification (LAMP) assay is presented, utilizing the high amplification efficiency of the loop-mediated isothermal amplification (LAMP) method
We selected a colorimetric sensing strategy based on the non-crosslinking aggregation model of AuNPs and oligonucleotide-functionalized AuNPs (DNA-AuNP probes) promoted by pH- and salt-induced aggregation
Summary
In the field of pathogen diagnostics, innovative point-of-care compatible methods such as colorimetry have become increasingly important in recent years. Other groups developed modules that are based on digital image colorimetry (via smartphone) [33] or make use of a diffraction grating and image processing to measure the particle size and concentration [34], or use a tungsten-filament lamp with optical fibers and a commercial portable spectrometer [35] Multichannel spectrophotometers, those based on diode arrays and custom-made detection cells [36], present many advantages, such as spectrum acquisition in a very short interval of time, good resolution and accuracy in wavelength measurements, and adequate sensitivity and dynamic range for many applications. The development of a simple, hand-held, and low-cost device for carrying out colorimetric detection based on plasmonic nanoparticles and a DNA-amplification (LAMP) assay is presented, utilizing the high amplification efficiency of the LAMP method This assay, with a simple assay design and simple readout device requirements, can be monitored in real-time on-site and allows thereby rapid testing for different analytes
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
