Abstract
The confocal laser scanning microscope (CLSM) is a device for obtaining high-resolution optical images of immunofluorescent staining. The CLSM can produce in-focus images of thick specimens, a process known as optical sectioning. The images are reconstructed with a computer, using 3-dimensional image software, allowing 3-dimensional reconstructions of topologically complex objects. On the same tissue sections, the CLSM can obtain the images of differential interference contrast. Recently, a special inverted CLSM-the multimode microscopy system-has been used to examine the morphology and functions of cells. A multimode microscopy system can be used to obtain images of CLSM, total internal reflection fluorescence, time-lapse, and micromanipulation. In the present study, we show images of pancreatic cancer cells as an example.
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