Abstract
In this paper, we conducted an assembly of microstructures made of a thermoresponsive gel using hysteresis character of the thermoresponsive polymer solution. This method can be used for 3 dimensional cell assembly by embedding cells in the thermoresponsive gel structures. Gel sheets can be maintained the gel condition by the hysteresis character and the microstructures can be formed by assembling the gel sheets. The generation of thermoresponsive gel was conducted using microheaters which were fabricated on a substrate. The fabrication of a gel sheets was achieved using the hysteresis character and the fabricated gel sheets were picked and placed by the probe device. The positioning of the gel sheets can be precisely controlled by the micromanipulator to manipulate the probe. The results indicate the method we propose has a great possibility to achieve 3D cell assembly without large stress to cells during the assembly and cell culture.
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