Abstract
The phiC31 integrase is a site-specific recombinase originally isolated from a Streptomyces bacteriophage. It has been shown by our lab to perform a recombination reaction in mammalian cells that can result in the efficient chromosomal integration of plasmid DNA and long-term transgene expression. Such naked DNA vectors have a wide range of possible uses, including many gene therapy applications. Traditional plasmid vectors have been associated with short-lived expression, and viral vectors have been shown to cause toxicity. However, these are problems that an integrase-mediated non-viral gene therapy has the demonstrated potential to overcome.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
