Abstract
Misidentification of Brucella species from clinical specimens using commercial bacterial identification systems is a recurring problem. An isolate from a bacterimic patient was identified as Bergeyella zoohelcum by MicroScan Walk-Away (Siemens Healthcare Diagnostics Inc., West Sacramento, CA, USA) and as Brucella melitensis by Vitek 2 system (bioMérieuxInc., Durham, NC, USA). Because of this identification ambiguity by the two automated bacterial identification systems we performed 16S rRNA sequencing and serotyping of the isolate and confirmed it as a Brucella spp. Combining the sequence data with the Vitek 2 system data we conclude that the infection was caused by B. melitensis.
Highlights
Brucella is a potential agent of bioterrorism and a significant pathogen causing laboratoryacquired infections [1,2]
Brucella species has been misidentified as Moraxella phenylpyruvica using the API 20NE system [5,6], Ochrobacterium anthropi by the API 20NE system [7] and RapID NF Plus system (Innovative Diagnostic Systems Inc., Atlanta, USA) [8], and as Haemophilus influenzae biotype IV and Moraxella species with use of MicroScan panels
We describe misidentification of Brucella melitensis as Bergeyella zoohelcum by the MicroScan Walk-Away system (Siemens Healthcare Diagnostics Inc., West Sacramento, CA, USA)
Summary
Brucella is a potential agent of bioterrorism and a significant pathogen causing laboratoryacquired infections [1,2]. The confirmative diagnosis of brucellosis is made by isolation of the pathogen from blood and other representative specimens. We describe misidentification of Brucella melitensis as Bergeyella zoohelcum by the MicroScan Walk-Away system (Siemens Healthcare Diagnostics Inc., West Sacramento, CA, USA).
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