Abstract
This chapter discusses a chemical approach to the mapping of T-cell epitopes in proteins. Mapping of T-cell epitopes is the first step in elucidating the fine specificity of the T-cell clones and in characterizing the residues interacting with the major histocompatibility complex (MHC) proteins. T-cell epitopes are composed of continuous protein fragments having 10–15 amino acid residues. Different methods have been used to identify T-cell epitopes: (1) treatment of the proteins with specific enzymes or chemicals followed by purification to homogeneity of the stimulatory peptide by high-performance liquid chromatography or similar methods, (2) use of natural or recombinant protein fragments, (3) chemical synthesis of the entire protein, and (4) use of algorithms to localize probable epitopes followed by chemical synthesis.
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