128-POS

Abstract

Objectives The purpose of the study is to determine the role of curcumin in the alteration levels of IL-1α, IL-6, and TNF-α as pro-inflammatory cytokines in monocyte culture exposed to preeclamptic plasma, as well as the effect to the transcription factors; NF-kB and PPAR-γ. Methods Plasma was taken from preeclamptic women (n = 12) and normotensive pregnant women (n = 12). Monocyte cultures were taken from non-pregnant healthy woman. Monocyte cultures were incubated with plasma for 48 h. Curcumin in various doses were given in monocytes culture before and after preeclamptic plasma exposure. The level of IL-1 α , IL-6, and TNF- α as well as NF-kB and PPAR- γ in each culture were determined by ELISA procedures. The final data was analyzed by ANOVA and PATH Analysis. Results This study shows a significant increase (p-value 0.05) in the levels of pro-inflammatory cytokines (IL-1 α , IL-6, and TNF α ) in monocyte cultures exposed to preeclamptic plasma compared with normotensive pregnancy plasma. Curcumin treatment in various doses could decrease significantly (p-value 0.05) pro-inflammatory cytokines levels in monocyte cultures that has been already stimulated by preeclamptic plasma. After curcumin treatment, there was decreased level of nuclear NF-kB p50 and increased level of PPAR- γ significantly. Curcumin have direct effect to decreasing level of nuclear NF-kB p50 and also curcumin indirectly influenced to the level of nuclear NF-kB p50 by the increased level of PPAR- γ . Conclusions Curcumin could decrease levels of pro-inflammatory cytokines (IL-1 α , IL-6, and TNF α ) in monocytes culture exposed to plasma preeclamptic by affecting transcription factors NF-kB and PPAR- γ . Curcumin has potential advantage in the prevention and future treatment of preeclampsia, through inflammation pathway assumed as responsible pattern to the development of preeclampsia. Disclosure B. Rahardjo: None.