Abstract
Type-α transforming growth factor (TGFα) is a mitogenic peptide factor produced extracellularly by tumor cells and by virally and chemically transformed cells in culture. TGFα is obtained from the natural source, and the synthesis of TGFsα provides sufficient material to enable more extensive studies on their mode of action and the role of their structures on their transforming activity. This chapter discusses the methods necessary to achieve the manual synthesis of rTGFα. The chapter also discusses several of the latest improvements of the solid-phase method, particularly the novel low-high HF to minimize strong acid-catalyzed side reactions. Some of the salient features and rationale for the improved version of the solid-phase method for the chemical synthesis of rTGFα are stable peptide–resin linkage, maximal and stable side chain protection, quantitative monitoring of the coupling step, and a new deprotection strategy: the low-high HF cleavage method.
Published Version
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