12] Atomic absorption spectrometry

Abstract

Publisher Summary Atomic absorption methods are the most widely used for the determination of metals in biological materials. This chapter discusses an overview of the field to help in the selection among the available techniques. The various elements will then be treated with recommendations for preferred methodology. This chapter discusses the understanding that atomic absorption is two techniques: flame atomic absorption (AAS) and furnace AAS. Flame AAS is generally the best analytical technique available for samples that are most easily collected as solutions, if the analyte concentration is above the mg/liter range in the solution. Precision of about 1% is routinely obtained and better precision is available if extra care is taken in the preparation of standards and if slightly more time-consuming methods are used. There are several problems that stand in the way of increased utilization of furnace AAS for trace metal analyses. More importantly, many analysts fail to give adequate attention to the laboratory hygiene that is required at the very low levels accessible by furnace AAS.