Abstract
The strongest risk factors for developing HNSCC are human papillomavirus infection and alcohol/tobacco use. The latter gives rise to a clinically aggressive tumor with a poor prognosis, creating an urgent need for effective screening strategies. Exosomes, or small extracellular vesicles (sEV), and their content are emerging as potential cancer biomarkers. Here, CD9/63/81 positive sEV were isolated from circulating plasma of HNSCC patients and healthy volunteers by immunoaffinity purification and characterized by Western blot and Nanoparticle Tracking Analysis.
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