Abstract

The release of barley genome sequences will accelerate the genomic and genetic research in barley. Using next-generation sequencing (NGS) technology, it is possible to re-sequence entire barley genomes or transcriptomes more efficiently and economically than ever before. The chapter covers barley genetic and physical maps, and barley genome sequences. We summarized NGS sequencing technologies and their applications in genetic marker development, QTL mapping, gene cloning, transcript profiling identification, and epigenetic mechanism discovery in barley. These technologies will provide efficient tools to explore barley germplasm and improve breeding efficiency.

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