Abstract
Viruses protect their genome and after endosome escape can disassemble to release their genetic contents. These two functions of protection and triggered-release are competing functionalities that have been difficult to engineer into nonviral transfection reagents. In order to mimic viral condensation of DNA, packaging and protection, and triggered release, we have designed a novel photolabile monomer, P25M, for plasmid DNA (pDNA) delivery. P25M was synthesized via a 6-step reaction scheme and consists of three functional domains: 1) a cationic domain of 25 kDa polyethylenimine (PEI) to electrostatically condense the pDNA; 2) a polymerizable domain (a methacrylamide moiety) for crosslinking the pDNA within the polyplex; and 3) a photolabile nitrobenzyl domain for triggered release by 365 nm light (Fig.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.