Abstract
This study is unrefined ZnO, nanoparticles is expose M4 medium to search nanoparticle aggregation and Daphnia magna was any effect by immobilization and mortality. ZnO and nanoparticle powder-size is respectively 20 nm and 40 nm. but, M4 medium has about respectively as 1333 nm and 1628 nm, 40 to 70 times were agglomerated. Immobilization of ZnO and nanoparticles was influenced both time and concentration the higher to swimming of D.magna. Especially, The immobilization of D.magna in nano-ZnO is greater than that influence in nano-. Mortality of ZnO nanoparticle is higher rate at long time and high concentration. nanoparticle observed mortality at 10ppm concentration after 72h. Consequently, when Nanoparticles is introduced into ocean. Particle size become grow. Additionally, aggregation be caused affect aquatic ecosystems.
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