W oodsholea

Abstract

Abstract Woods.ho'le.a. N.L. fem. n. Woodsholea , named in honor of the Woods Hole Oceanographic Institution, Massachusetts, USA. Proteobacteria / Alphaproteobacteria / Rhodobacterales / Hyphomonadaceae / Woodsholea Woodsholea species are Gram‐negative cells, rod shaped, and dimorphic; one type of cell possesses a prosthecum (stalk), extending from one pole as a continuation of the long axis of the cell. Adhesive material is present at the distal end of the stalk attaching the cells to interfaces. The other type of cell is mobile by means of a polar flagellum. Multiplication by binary fission. Colonies are circular, convex, and colorless. Chemoorganotrophic aerobes, and cells can store carbon as poly‐β‐hydroxybutyric acid. Species grow on peptone/yeast extract media supplemented with NaCl. The growth temperature range is 20–40°C, and optimal pH for growth is approximately neutral. Do not reduce nitrate. Cells are positive for alkaline phosphatase, naphthol‐AS‐BI‐phosphohydrolase, leucine arylamidase, acid phosphatase, esterase (C4), esterase lipase (C8), oxidase, and trypsin, but negative for α‐ and β‐galactosidase, α‐glucuronidase, α‐ and β‐glucosidase, α‐mannosidase, and α‐fucosidase. The genus is characterized by two major fatty acids, 18:0 and 18:1 ω7 c , and minor amounts of 12:0 3‐OH, 16:0, 17:0, and the unidentified fatty acid ECL 15.275. The polar lipids are α‐ d ‐glucopyranosyl diacylglycerol, α‐ d ‐glucopyranuronosyl diacylglycerol, sulfoquinovosyl diacylglycerol, and α‐ d ‐glucuronopyranosyl diacylglycerol taurine amide. Isolated from sea water. DNA G + C content (mol%) : 55.7 (genome sequence). Type species : Woodsholea maritima Abraham, Strömpl, Vancanneyt, Bennasar, Swings, Lünsdorf, et al., 2004, 1233 VP .