Abstract
The phosphoaminolipids obtained from cephalins of the muscle of a tuna, Thynnus orientalis, were converted to their dinitrophenyl (DNP) derivatives and applied to the chromatographic separation with a silicic acid/celite (2:1) column and chloroform/methanol to yield a DNP-ethanolamine-containing lipid (I), and amino acid-containing lipids (II), which gave a DNP-serine-containing lipid (II-a) and a mixture of DNP-serine-containing and DNP-threonine-containing lipids (II-b) by rechromatography. Hydrolysis of I with hydrochloric acid yielded DNP-ethanolamine, which gave free ethanolamine by decomposing with concentrated ammonium hydroxide. DNP-serine and-threonine, and free serine and threonine were isolated and identified with the same manner from II-a and II-b respectively. Periodate oxidation of the threonine fraction obtained from DNP-threonine of II-b gave acetaldehyde and glyoxylic acid. Component fatty acids of each fraction were found to be palmitic and oleic acids by paper chromatography. The presence of palmitoyloleoylglycerylphosphoryl esters of ethanolamine, serine and threonine can be supposed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
