Abstract

The resistance of S. parasitica aganist malachite green was variable according to its life-time; i. e. the growth of hyphae was restrained completely in pepton-solution with 0.02 ppm., and the formation of zoosporangia or gemmae, and the germination of resting spores were checked with 0.2 ppm. (Fig. 1, Table 3) The correlation diagram between the concentration of M. G. and the time need to kill the hyphae or sporangia on the eggs of Hypomesus oliaus was shown as a straight line when both factors were expressed in logarithum (Fig. 2). As the infection of the disease could not be checked unless the formation of zoospore ceased, it may be safely said that the disease is carried chiefly through zoospores (Table 3). From these experiments, it is suggested that in practice controling the disease, it may be enough to treat the trout eggs for about 20 minutes in 5 ppm. of M. G., and in the case of the eel it may be necessary to use more concentrated M. G. solution than 0.2 ppm.

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