Abstract
To select the most proper soil for root initiation from stem cuts of Birdsfoot trefoil (Lotus corniculatus L.), eight-week-old stem cuts were cultured on three types of soil [commercial bed soil, decomposed granite (DCG), and river sand] for one month. The results showed that the root initiation ratios on DCG (77.8%) and river sand (70.0%) were relatively high, but the ratio on commercial bed soil (41.1%) was very low. To examine the effect of rare earth (RE) and Indole-3-Butyric Acid (IBA) on root initiation from stem cuts of Birdsfoot Trefoil, stem cuts were cultured on two types of soil (DCG and river sand) with treatment of RE and IBA for one month. The root initiation ratios turned out to be 90.0% (DCG with 60 ppm of RE), 80.0% (river sand with 20 ppm of RE), 96.7% (DCG with 40 ppm of IBA), and 96.7% (river sand with 40 ppm of IBA). These results suggested that the most efficient way for root initiation of Birdsfoot trefoil was to culture the stem cuts on river sand or DCG over 30 days with IBA treatment (40 ppm).
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