沉默 c2orf68 基因对结直肠癌细胞增殖的影响

Abstract

Objective: To explore the c2orf68 gene expression of human colorectal adenocarcinoma SW480, SW620 and LS174T cell lines. Further more,to observe the change of their biological characteristics in three cell lines by applying RNA interference technique to silence c2orf68 and to clarify its in the pathogenesis of colorectal cancer. Methods: Using siRNA online design software designed and synthesised of siRNA interference fragments, and transported them to the three cell lines. The mRNA expression of the c2orf68 were detected by RT-PCR,the cell proliferation was checked by MTT,and the cell apoptosis was measured by flow-cytometry. Results: The mRNA expression of the c2orf68 was inhibited by the two pairs siRNA in SW480,SW620 and LS174T cell lines. It showed that the inhibition rates were as follows: 50. 05%,50. 05%; 49. 69%,49. 69%; 50. 15%,45. 79%. Then,siRNA1 of high interference efficiency was selected. The cell proliferation inhibitory rate was 21. 2% in LS174T cell line( P 0. 05). The cell apoptosis rate was 18. 13% in LS174T cell line( P 0. 05). Conclusions: The successfully obtained siRNA fragement which specifically degraded its c2orf68 mRNA,inhibited the growth of the test cells,promoted cell apoptosis. It indicated that c2orf68 might be associated with the proliferation on colorectal cancer cells,its pathogenesis might be through gene expression,further regulated the change of the corresponding biological characteristics,and caused the occurrence of colorectal cancer.