Abstract
Objective - a comparative analysis of the frequency of polymorphism 4G/5G gene PAI-1 inthe Chernivtsi region residents who suffer from various forms of ulcer disease andassessment of the possible relationship between different genotypes and the developmentof complications.Material and methods. 60 patients with ulcer disease: 42 (70%) men and 18 (30%)women aged 21 to 83 years. Duodenal ulcer has been found in 37 (61.67 %) patients, inthe rest (38.33%) - ulcer of the the stomach. Uncomplicated ulcer was found in 12 (20%)patients, ulcer perforation - in 5 (8.33%), 43 (71.67%) patients had ulcer complicatedby acute bleeding. Recurrence of bleeding occurred in 14 (32.56%) patients. PAI genotyping with 4G/5G polymorphism has been performed by a polymerase chain reaction.In order to do that, a common genomic DNA has been isolated from the blood of probands, using a set of reagents to isolate DNA from the clinical material "DNA-sorbent B".Results. 33.33% of the examined patients with ulcer disease without any acutecomplications have a homozygous genotype 4G / 4G for 5G4 polymorphism of the РАІ-1gene, and 66.67% - the genotype 4G / 5G; in no other case the homozygous genotype 5G/ 5G has been detected. 60% of patients with perforation of the ulcer have the genotype4G/4G, and 20% - other variants of polymorphism. A homozygous genotype 4G/4G(13.95%) occurs the least among patients with acute ulcerative bleeding, and the number of carriers of the allele 5G (genotype 5G / 5G and 4G/5G) statistically significantlyprevails among patients with perforation of the ulcer (p=0.03, =6.23) and ulcerwithout bleeding (p=0.02, =5.32). The 5G allele occur somehow frequently, thoughunreliable, in patients with recurrent bleeding than in patients without those.Conclusions. Among patients with ulcer disease without acute complications of theChernivtsi region residents 33.33% have homozygous genotype 4G / 4G polymorphism4G / 5G gene PAI-1, and 66.67% - genotype 4G/5G; in no case a homozygous genotype5G/5G has been found. 60% of patients with ulcer perforation have the 4G/4G genotype,and 20% - the 4G/5G and 5G/5G genotypes. A homozygous genotype 4G/4G (13.95%)occurs the least among patients with acute ulcerative bleeding, and the number ofcarriers of the allele 5G (genotype 5G / 5G and 4G/5G) statistically significantlyprevails among patients with perforation of the ulcer (p=0.03, =6.23) and ulcerwithout bleeding (p=0.02, =5.32). At the same time, the 5G allele occurs somehow frequently, though unreliable, in patients with recurrent bleeding than in patients withoutthose, which confirms the role of hereditary disorders of the РАІ-1 gene in the development ofulcerative hemorrhages. Taking into account the 4G/5G polymorphism of the PAI-1 gene canbecome a component of the complex for predicting the occurrence of ulcerative bleeding inclinical conditions.
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