Abstract
Based on the gold nanorods modified by 11-mercaptopropanic acid( MUA) as the skeleton,the low molecular weight polyethylenimine PEI was conjugated to the surface of GNRs / MUA to form GNRs / MUA / PEI nanocomplexes. Firstly,surface modification of gold nanorods was carried out by using MUA to reduce the toxicity of gold nanorods caused by CTAB surfactants,and then further modified by low molecular weight PEI. The large surface area of gold nanorods allows them to carry more genes,which can simultaneously reduce the toxicity of cationic polymer and improve the transfection efficiency of the whole system. GNRs / MUA / PEI were characterized by transmission electron microscopy( TEM),UV-Vis absorption spectra,and Zeta potential. The results show that MUA and PEI are conjugated to GNRs successfully which cause charge reversal on the surface of GNRs,and the optical properties of GNRs are well preserved. In vitro cytotoxic effects of GNRs / MUA / PEI( 1. 8 k Da) are quantified by MTT assay,and the results suggest that the cell viability is above 75%at the concentration of 300 μg / m L,much higher than the commercialized PEI( 25 k Da).
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