酵母Ｔｗｏ‐ｈｙｂｒｉｄ法を用いた環境試料のエストロゲン様活性評価に関する研究

Abstract

A number of bioassays for screening the compounds for estrogenic activity have been proposed so far, however, a yeast two-hybrid system appears more simple and inexpensive assay with high repeatability. The biggest problem of this bioassay is that it cannot measure estrogenic activity in some of the environmental samples. At is was assumed that the co-existing compounds were responsible for these false negatives, two methods were tested:1. Separation of such co-existing chemical interferences by use of liquid chromatographic method.2. Masking the retarding effect of such co-existing chemicals interferences by the addition of S9mix.The results indicated that both methods were effective to identify the estrogenic activity, however, the addition of S9mix was much more simple to conduct and more effective to determine the estrogenic activity in environmental samples.