Ｐｒｏｌｉｆｅｒａｔｉｎｇ ｃｅｌｌ ｎｕｃｌｅａｒ ａｎｔｉｇｅｎ（ＰＣＮＡ）による悪性リンパ腫の検索

Abstract

Proliferative activity of neoplastic and non-neoplastic cells in 123 lymphoma cases [non-Hodgkin's lymphoma (NHL) 70 cases, Hodgkin's disease (HD) 53 cases], as well as 18 control reactive lymphadenopathies, was estimated by employing immunohistochemical procedures [avidin-biotin complex (ABC) method] with a monoclonal antibody against proliferating cell nuclear antigen (PCNA) which is synthesized during the late G1 and S phases. Immunostaining of PCNA was achived using sections of formalin fixed and paraffin embedded lymphoma tissue stored in the pathology files of our departments snd of some affiliated institutions. PCNA positive rates (positive cells/total cells) were determined, and relationship between these rates and pathological parameters were investigated. There was a positive correlation between the PCNA positive rates and a Working Formulation in NHL (P<0.05). In HD, Reed-Sternberg (RS) cells were highly positive (44%). No relationship was found, however, between the PCNA positive rates and a Rye classification. This finding may be explained either by speculating the cell cycle of RS cells derranged in a manner that G1-S phases are prolonged or, altematively, that proliferated RS cells destroyed by host reaction. These results indicate that PCNA immunostain is a useful method to analyse malignant lymphomas in formaldehyde fixed, paraffin embedded materials.