Удосконалення аналітичного визначення залишкових кількостей діючих речовин класу триазолів в соках

Abstract

Today, the most common among fungicides approved for use in Ukraine, as well as in other countries, are compounds of the triazole class. Juices, as a product of processing agricultural raw materials, can be consumed by a wide range of the population. The purpose of work was to develop analytical methods for compound of triazole (difenoconazole and propiconazole) in juices to ensure quality control of agricultural products grown with the use of these fungicides. Conditions for chromatography of difenoconazole and propiconazole using gas-liquid chromatography with capillary column SH-Rxi-5ms (30 m 0,25 mm) and electron capture detector were determined. Optimal conditions of difenoconazole chromatography were established: thermostat column temperature - 280 C, evaporator temperature - 290 C, detector thermostat temperature - 300 C. Retention time: isomer 1 - (6,91 +- 0,1) minutes, isomer 2 - (7,04 +- 0,1) minutes. The linear range of detection is 0,01-0,04 ug/cm3. The optimal conditions for chromatography of propiconazole were also established: the temperature of the column thermostat in the programming mode: initial T - 100 C with an exposure of 1 minute; from 90 C to 260 C - rate of temperature rise 40 C/min; exposure - 7,45 minutes; evaporator temperature - 270 C, detector temperature - 280 C. The retention time under these conditions was 3,49 +- 0,1 minutes for isomer 1 and 3,57 +- 0,1 minutes for isomer 2. The linear range of detection is 0,01-0,05 ug/cm3. The calibration dependence of the area of peaks of the investigated substances on their concentration was established and described by the linear regression equation.