Abstract
The injured or dead thalli of Monostroma are rather difficult to distinguish from the normal ones either by naked eye or by microscopic observation. Vital staining with methylene blue, neutral red, toluidine blue or rhodamin B gives also an unsatisfactory result. Comparing the spectral absorption curves of whole thalli, increased grade of injury is accompanied by lowering of absorbance over the visible range including the specific peaks of chlorophyll and carotene. Ethanol extracts of such thalli also give changes in spectral absorption nearly in parallel with the thalli themselves. The treatment of thalli with a tetrazolium salt (TTC) gives a satisfactory result corresponding to the grade of injury of cells. But it needs to use a rather high concentrated solution of TTC (4mg./cc), to evacuate sufficiently, and to incubate at 30°C. adequately.
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