Abstract
Citrus Huanglongbing (syn. greening disease) caused by 'Candidatus Liberibacter asiaticus' were surveyed on the Amami islands from 2002 to 2006. A total of 212, 587 Rutaceae plants were surveyed at 29, 063 sites on the Amami islands. Samples that showed a greening-like symptom (17, 384 samples) were examined by a polymerase chain reaction (PCR) assay according to the method of Jagoueix et al. (1994). Of these, 1, 041 (0.5%) samples were identified as being infected with 'Candidatus L. asiaticus.' These infected samples were found on the Kikai-jima, Tokuno-shima, Okierabu-jima, and Yoron-tou islands; however, infected samples were not found in Amami-O-shima (Amami main island).
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