Характеристика выживаемости штаммов E. coli K-12 и генно-модифицированного E. coli K-12 SGM2.0Pyc-int в природных и антропогенных образцах воды и почвы

Abstract

The survival time of the E. coli K-12 strain and the genetically modified E. coli K-12 SGM2.0pyc-int strain developed on its basis, a producer of succinic acid, has been determined in various natural and anthropogenically contaminated water and soil samples. The dynamics of the abundance of both strains in water samples from a well, a pond and a sewer collector was evaluated. It was found that within 10 days of the experiment, the cells of both strains died in all water samples. The highest dynamics of cell death, within 4 days, was observed in the wastewater from the collector. The survival rate of both strains in the same environment was similar; however, there was a tendency towards lower viability of the genetically modified producer. In the sod-podzolic and urbanozem soil samples, the growth of the studied strains was suppressed regardless of the type of soil. The absence of viable E. coli K-12 and E. coli K-12 SGM2.0pyc-int cells was observed after 10 and 7 days of incubation, respectively. The results indicate a high level of safety of the genetically modified E. coli K-12 SGM2.0pyc-int strain and the lack of its competition with microflora of water and soil samples from natural and anthropogenic sources. Escherichia coli K-12, genetically modified microorganisms, survival, natural ecosystems, environmental monitoring The authors are grateful to the Russian State Collection of Industrial Microorganisms National Bio-Resource Center (BRC VKPM), State Research Institute for Genetics and Selection of Industrial Microorganisms, for providing bacterial strains. We thank A. D. Novikov, a researcher of the Kurchatov Genomic Center - GosNIIgenetica, for participation in the planning of experiments and discussion of the results and Zakharova M.V., Associate Professor of the Department of Theory of State and Law of the O.E. Kutafin University (MGUA) for legal advice in the preparation of regulatory and legal documentation on genetic safety. The work was carried out with the financial support of the Russian Foundation for Basic Research (project no. 18-29-14005).