Исследование ассоциаций полиморфизма гена SCD с содержанием внутримышечного жира у свиней пород дюрок и крупная белая

Abstract

Abstract. A scientific novelty is the study of the allele pool of domestic reproduction pigs, the determination of population genetic parameters by DNA markers, and the search for associations of genetic variants with economically significant traits. The aim of the work was to study the influence of SCD Y487830 polymorphism: g.2228 T > C to the IMF content and to search for associations of genotypes with economically significant traits in domestic reproduction pigs. The studies were conducted in 2019 on pigs reared in Selection and Hybrid Center LLC (Voronezh region). Genotyping were determined by the QuantStudio™ 5 Real-Time PCR System using the developed test system. The content of IMF (%), backfat thickness (mm), lean (%), live weight (kg) in 103 boars and 27 gilts Duroc pigs, 30 Large White boars was determined using an ExaGo ultrasound scanner. The main diet and animal welfare conditions were identical and corresponded to zoosanitary standards. Model equation for multivariate analysis of variance with interaction was used to evaluate the effect of genotype by SCD on productive qualities. The frequency of the T allele and TT genotype in the group of Duroc pigs was 0.419 and 12.7 %, respectively. Large White pig breeds were characterized by the frequency of the T allele – 0.686, TT genotype – 46.8 %. All Landrace pigs were carriers of the TT genotype. The superiority of the C allele carriers in the content of IMF was shown. The difference between Duroc boars with TT and CC genotypes was 0.42 % (P ≤ 0.05), and individuals with CC genotype exceeded TT genotype carriers in the backfat thickness of more than 3 mm (P ≤ 0.001). The results confirm the association of the SCD genotype with the content of intramuscular fat and the backfat thickness in the Duroc boars.