Abstract
Specific features of embryonic development of eggs isolated from gonads of Trichuris skrjabini nematodes females, obtained during helminthological opening in sheep, were studied. It is proved that during the growth and development of this species of trichurises eggs there are significant changes in the metric indices of their length and width, as well as in the length and width of their lids. It is determined that at 27 °С in laboratory conditions T. skrjabini eggs become infectious during 51 days with survival rate of 80.0±0.82 % (20.0±0.81% of eggs stop in development and subsequently die). The embryogenesis process has six morphologically distinct stages: protoplast (from the 1 st to the 12 th day); blastomeres formation (from the 6 th to the 24 th day); bean-like embryo (from the 12 th to the 33 d day), tadpole-like embryo (from the 15 th to the 36 th day); larval formation (from the 27 th to the 48 th day); and mobile larva (from the 27 th to the 51 st day). The development of T. skrjabini eggs to the infectious stage in laboratory conditions is characterized by their lengthening to 75.7±0.36 μm (by 2.0%, р < 0.05), narrowing to 37.3±0.30 μm (by 2.4%, р < 0.05) and lengthening to 12.2±0.42 μm (by 16.4%, р < 0.01) and thinning to 12.1±0.10 μm (by 5.5 %, р < 0.01) of the egg lids. The changes of egg shell thickness were not statistically significant. Considering the data on the morphological structure and the period of nematodosis pathogens eggs development it is possible to plan the time of taking measures to prevent and control trichurosis in sheep.
Highlights
Сегодня овцеводство, как и животноводство в целом, приобретает весомое социально-политическое значение
Вместе с тем А.Г. Гареев установил
Specific features of embryonic development of eggs isolated from gonads of Trichuris skrjabini nematodes females
Summary
100% яиц, полученных из гонад самок, были на стадии протопласта (рис. а). Начиная с 3-го и по 9-й день культивирования эмбриогенез характеризовался переходом первой стадии во вторую – активным образованием и дроблением бластомеров в яйце. 100% яиц, полученных из гонад самок, были на стадии протопласта Уже с 9-го дня эксперимента 2,0% яиц содержало морулу, которая морфологически похожа на боб – стадия бобообразного зародыша. Начиная с 15-го дня культивирования, у 6,7±0,71% яиц T. skrjabini наблюдали морфологическое преобразование бобообразного зародыша в головастикообразный. Максимальное количество яиц в этой стадии выявляли с 24-го (46,3±0,62%) по 33-й (58,7±0,83%) день эксперимента. Стадии эмрионального развития яиц нематод овец Trichuris skrjabini при экспериментальном культивировании (× 400): а – протопласта; б – формирования личинки. Похожие исследования морфометрических показателей яиц нематод в процессе их эмбриогенеза были проведены при культивировании яиц, выделенных из гонад самок гельминтов. Таблица 2 Морфометрические показатели яиц, выделенных из гонад самок нематод овец Trichuris skrjabini, при эмбриональном развитии, M±m (n = 10), мкм
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