Клональне мікророзмноження імбиру в умовах in vitro

Abstract

The purpose. To develop a method of clonal microreproduction of ginger for deriving quality planting stock. Methods. Laboratory, biotechnological, statistical. Results. Germination of gemmas on roots in conditions of thermostat at temperature 28±2°C and moisture content of 90% has been observed for 4 weeks of cultivation. Yield of gemmas has made 161,8%. Application of 0,1% of solution of sublimate with exposure of 45–50 min ensures 50,0–63,9% of aseptic gemmas of ginger at different biotypes. Altitude of primary shoots in a month of cultivation averaged 2,2 cm, amount of roots — 4,5 pieces for 1,9-cm gemma. Addition into nutrient medium of BAP raises sprout-formation up to 7,7 pieces/shoot for 1 passage. Formation of roots was observed on 10–14 day of cultivation in all probed biotypes irrespective of presence of auxins in nutrient medium. In conditions of growing of microplants of ginger in vitro survival of seedling made 72–98%. Conclusions. At stimulation of sprout-formation on ginger hands in thermostat at temperature 28±2°C and moisture content of 90% the yield of gemmas has made 161,8%. Application of 0,1% of solution of sublimate with exposure of 45–50 min enables to gain 50,0–63,9% of aseptic gemmas of ginger at different biotypes. The yield of viable gemmas makes 61,4–81,9%. Application of BAP (3 mg/l) ensures high multiplication ratio of ginger — up to 7,7 pieces/shoot for 1 passage. Culture shoots of ginger in vitro do not demand add-on of auxins into nutrient medium for stimulation rhizogenesis. Culture seedling of ginger in vitro has high level of survival in conditions of open ground — 72–98%. That enables to use a method of cloning in vitro for deriving quality planting stock. The developed method of clonal microreproductions of ginger enables to obtain from one in vitro explant up to a thousand rooted plants.