Abstract
A polyA-containing thyroglobulin mRNA (mRNAptf) was isolated from human thyroid in health and congenital goiter. A singlestranded complementary DNA (cDNA) was synthesized on a polyA-containing mRNA Tl [ matrix by reverse transcription in the presence of RNAse inhibitor from human placenta; this cDNA was used in hybridization experiments for measurements of mRNA. The amount of mRNApH in the presence of TH deficiency was normal in the examined goiter case, indicating an impaired TH gene expression at the translation level.
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