Abstract
In order to preserve the gene pool of medicinal plant Oxytropis lanata (Pall.) DC. we analyzed allozyme polymorphism and identified reliable and informative marker enzyme systems of this species; also we studied the response of seeds to deep freezing in liquid nitrogen (–196 ºС). Population has an average level of polymorphism (P95 = 41,2 %, P99 = 52,9 %, A = 1,58, Ho = 0,158, He = 0,171) in general typical for herbaceous legumes, and can serve as a source of material for gene pool conservation of the species. Deep freezing has not led to the death of the seeds; it was marked stimulatory effect of ultralow temperatures, expressed as an acceleration of germination and sharp increase of germinability (98,6 ± 2,3 %) compared to the control (12,0 ± 3,5 %) that is associated with overcoming physical dormancy. There were no abnormalities in the development of seedlings from seeds passed cryopreservation.
Highlights
In order to preserve the gene pool of medicinal plant Oxytropis lanata (Pall.) DC. we analyzed allozyme polymorphism and identified reliable and informative marker enzyme systems of this species; we studied the response of seeds to deep freezing in liquid nitrogen (–196 oС)
Population has an average level of polymorphism (P95 = 41,2 %, P99 = 52,9 %, A = 1,58, Ho = 0,158, He = 0,171) in general typical for herbaceous legumes, and can serve as a source of material for gene pool conservation of the species
Deep freezing has not led to the death of the seeds; it was marked stimulatory effect of ultralow temperatures, expressed as an acceleration of germination and sharp increase of germinability (98,6 ± 2,3 %) compared to the control (12,0 ± 3,5 %) that is associated with overcoming physical dormancy
Summary
Примеч.: * – кодовый номер фермента (шифр) согласно изданию «Enzyme Nomenclature [Номенклатура ферментов]» (1979). Для анализа динамики прорастания учитывали начало прорастания и определяли показатели Т0 – число суток до начала прорастания и Т50 – число суток, в течение которых всхожесть достигала 50 % от итоговой всхожести. Глубокое замораживание проводили путем прямого погружения семян, завернутых в алюминиевую фольгу, в жидкий азот (–196 oС), где они хранились в течение 100 сут.; затем деконсервированные семена отогревали 2 ч. Семена O. lanata обладают физическим типом покоя, поэтому для изучения способов его преодоления проводили опыт по обработке семян концентрированной серной кислотой в течение 20 мин. Проросшие семена после криохранения и в контроле высаживали в контейнеры с почвой. В данном опыте в контроле использовали проростки из семян после скарификации, так как для них характерно дружное и одновременное прорастание. На I, III и IV этапе определяли сырой и сухой вес объединенной пробы из 20 проростков На I, III и IV этапе определяли сырой и сухой вес объединенной пробы из 20 проростков (табл. 5)
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