Abstract
Objective: To study the antioxidant activity and polyphenol content in Artemisia scoparia Waldst. & Kit., growing in the conditions of the Western Pamir. Methods: Materials for experiments served the plants Artemisia scoparia. Ground parts of A. scoparia collected from different heights and different slopes of the Western Pamir. Extracts were prepared from fresh leaves and stems of plants. Determination of polyphenol content performed using Folin-Ciocalteu reagents. The activity of antioxidants was determined by the ABTS method (2,2’-Azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid) analysis. Results: Analysis of the content of polyphenols in A. scoparia, growing in the conditions of the Western Pamir, showed that it varies from 80.2 to 100.0 μg/ml. So, in Darvaz (1200 m) the content of polyphenols was 90.4 μg/ml, Rushan (1800 m) – 90.0 μg/ml, Hoigal (3025 m) – 80.2 μg/ml, Sardem (3150 m) – 90.3 μg/ml, Bijondara (2351 m) – 100.0 μg/ml. The results show some indication of the height on the concentration of polyphenols in A. scoparia. Their highest content in plants was observed in Bijondara (100.0 μg/ml) at an altitude of 2351 meters. As for the activity of antioxidants, it was found that their number decreases depending on the height of growth of plants and varies from 140 to 220 μg/ml. Their highest activity was observed in plants collected in Vanj (200.0 μg/ml) and Darvaz areas (220.2 μg/ml) at an altitude of 1220-1430 m. At an altitude of 1800 m and higher, activity gradually decreased (160,1 μg/ml), amounting to 130.7-140.1 μg/ml at an altitude of 3150 m. Conclusion: The study showed that in the conditions of the Western Pamir, the height of growth has some influence on the accumulation of polyphenols in A. scoparia. In turn, the activity of oxidants decreases with increasing height above sea level. Perhaps this is due to the influence of stress factors of high mountains on the accumulation of biologically active substances. Keywords: A. scoparia, polyphenols, antioxidants, a height of growth, slopes, Western Pamir.
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