Abstract
본 ì°êµ¬ììë RBSDVì ì¸í¼ë¨ë°±ì§ P10ì ì½ëíë S10ì E. coliìì ë°íìì¼°ë¤. RBSDV-miryang isolate (GenBank JX994211)ë¡ë¶í° ì¶ì¶í ê²ë dsRNAì 주íì¼ë¡ S10ì í¹ì´ì ì¸ primer를 ì¬ì©íì¬ P10ì N-ë§ë¨ìì(1-834 nt, 1-278 aa)ì RT-PCRì ìí´ ì¦ííìë¤. ì¦íë RBSDV S10-N (1-834 nt)ì ë°í ë²¡í° pET32a(+)ì í´ë¡ëíì¬ E. coli BL21(DE3)ìì ë°íìí¨ í Ni-NTA affinity columnì¼ë¡ ë°íë ë¨ë°±ì§ì ì ì íìë¤. ì ì ë ë¨ë°±ì§ì ë©´ì ë물ì 주ì¬íì¬ ííì²ì ì ìíìë¤. ì ìë ííì²ì Western blot ë° ELISA ë¶ìì¼ë¡ RBSDVìì í¹ì´ì±ì íì¸íìë¤. 본 ì°êµ¬ìì RBSDV íêµ isolateì ííì²ì´ ì ìëìì¼ë©° ê¸í íì²íì ì°êµ¬ì ì¢ì ì¬ë£ë¡ íì©ë ì ìì ê²ì¼ë¡ 기ëíë¤.
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