ВЫДЕЛЕНИЕ И АНАЛИЗ ЭКСТРАКТИВНЫХ ВЕЩЕСТВ ЛАПЧАТКИ БЕЛОЙ (POTENTILLA ALBA L.), ВЫРАЩЕННОЙ В РАЗНЫХ УСЛОВИЯХ

Abstract

Potentilla alba L. is used as a medicinal plant that contains a variety of biologically active substances. Natural resources are not meeting the needs of the pharmaceutical industry. We got the first biomass plant regenerated P. alba in a hydroponic with micropropagation.We have conducted a comprehensive study of the chemical composition of a vegetable raw materials P. alba, prepared in the Department of Biotechnology (plants-regenerants) compared with those grown under field conditions (intact plants).We have established the quantitative content of the structural components in the samples: cellulose in roots and rhizomes of intact plants – 15,4%, in the roots of the plants regenerated – 4,3%, in the leaves of regenerated plants – 2,5%; lignin in roots and rhizomes of intact plants – 40,7%, in the roots of regenerated plants – 37,0%, in the leaves of regenerated plants – 36,8%.We determined the content of extractives in samples P. alba, recovered by sequential treatment with hexane, 96% and 40% ethanol solution, water and 1% aqueous sodium hydroxide solution. We identified a total content of extractives in the roots and rhizomes of intact plants – 15,3%, in the roots of regenerated plants – 11,2%, in the leaves of regenerated plants – 5,1%.We have characterized the chemical composition of extractives by UV-spectroscopy. Phenolic compounds (λ = 279,5-280,0 nm) are dominant in the composition of the extracts. We have established the authenticity of the samples by the TLC chromatography by the presence of flavonoids and tannins. We showed the identity of the phytochemical composition of vegetable raw materials produced by micropropagation and grown in a hydroponics for 2 months.