Abstract
The assessment of the quality of sea water samples was carried out according to biological indicators - the total number of microorganisms, which react sharply to changes in their environment. To ensure control over the ecological state of the aquatic environment, it is necessary to conduct regular monitoring studies on such microbiological indicators as the total number of microorganisms. Conventional microbiological methods for determining the total number of microorganisms are laborious and require a well-equipped laboratory and qualified personnel, and such analysis usually takes 24-48 hours. For a comparative assessment of the quality of sea water samples, the number of microorganisms was recorded by traditional methods and by the express method of chemiluminescence. As traditional methods, the method of inoculation on nutrient media, counting on a fluorescent microscope and on colored membrane filters were chosen. A chemiluminescent method was chosen for the rapid assessment of water by the total microbial abundance, which makes it possible to measure the content of bacterial cells by their ATP content. It is shown that the chemiluminescent method is superior to traditional methods for determining the total bacterial contamination (TBC, one of the main indicators of the natural object quality). At the same time, the chemiluminescent reaction made it possible to significantly reduce the measurement time and speed up data processing (measurement time did not exceed 2.5 min), i.e., it became possible to quickly analyze the samples under study in the field and in mobile laboratories. Direct positive correlations between the data determined by the traditional and new methods are revealed. The results obtained in the framework of a study on the determination of the number of microorganisms by classical methods and the chemiluminescence method allow us to recommend their use for express assessment of the quality of various natural objects.
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