Abstract

Some microorganisms isolated from drill sludge (Bacillus circulans NSh, B. firmus OBR 1.1, B. firmus OBR 3.1, Solibacillus silvestris OBR 3.2, B. circulans OBR 3.3, Halomonas sp. OBR 1, and Erwinia rhapontici OBR 4.1) have been shown to be capable of degrading crude oil, mineral oil, and diesel fuel. It has been established that the B. circulans NSh and B. firmus OBR 1.1 bacteria degrade 62 and 32% of crude oil, respectively, in an alkaline medium (pH 9) for 14 days and degrade 42 and 22%, respectively, in a saline medium (10% NaCl). It was demonstrated that under high salinity and alkalinity conditions the B. circulans NSh and S. silvestris OBR 3.2 bacteria effectively degraded petroleum hydrocarbons not only solely but also in combination with the crude-oil-oxidizing Dietzia maris AM3 microorganism. The efficiency of crude oil degradation by the B. circulans NSh + D. maris AM3 association was higher than that by each monoculture.

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